ausgewählte Publikationen

ausgewählte Publikationen

Proteomanalyse des Myelins

Jahn, O.; Siems, S.B.; Kusch, K.; Hesse, D.; Jung, R.B.; Liepold, T.; Uecker, M.; Sun, T.; Werner, H.B. The CNS myelin proteome: Deep profile and persistence after post-mortem delay. Front Cell Neurosci 2020; 14:239.

Siems, S.B.; *Jahn, O.; Eichel, M.A.; Kannaiyan, N.; Wu, L.M.N.; Sherman, D.L.; Kusch, K.; Hesse, D.; Jung, R.B.; Fledrich, R.; Sereda, M.W.; Rossner, M.J.; Brophy, P.J.; Werner, H.B. Proteome profile of peripheral myelin in healthy mice and in a neuropathy model. eLife 2020; 9:e51406 (*co-first author).

Erwig, M.S.; Hesse, D.; Jung, R.B.; Uecker, M.; Kusch, K.; Tenzer, S.; *Jahn, O.; Werner, H.B. Myelin: Methods for purification and proteome analysis. Methods Mol Biol 2019; 1936:37-63 (*co-corresponding author).

Joseph, S.; Vingill, S.; Jahn, O.; Fledrich, R.; Werner, H.B.; Katona, I.; Möbius, W.; Mitkovski, M.; Huang, Y.; Weis, J.; Sereda, M.W.; Schulz, J.B.; Nave, K.-A.; Stegmüller, J. Myelinating glia-specific deletion of Fbxo7 in mice triggers axonal degeneration in the central nervous system together with peripheral neuropathy. J Neurosci 2019; 39:5606-5626.

Erwig, M.S.; Patzig, J.; Steyer, A.M.; Dibaj, P.; Heilmann, M.; Heilmann, I.; Jung, R.B.; Kusch, K.; Möbius, W.; Jahn, O.; Nave, K.-A.; Werner, H.B. Anillin facilitates septin assembly to prevent pathological outfoldings of central nervous system myelin. eLife 2019; 8:e43888.

Patzig, J.; *Jahn, O.; Tenzer, S.; Wichert, S.P.; de Monasterio-Schrader, P.; Rosfa, S.; Kuharev, J.; Yan, K.; Bormuth, I.; Bremer, J.; Aguzzi, A.; Orfaniotou, F.; Hesse, D.; Schwab, M.H.; Möbius, W.; Nave, K.-A.; Werner, H.B. Quantitative and integrative proteome analysis of peripheral nerve myelin identifies novel myelin proteins and candidate neuropathy loci. J Neurosci 2011; 31:16369-16386 (*co-first author).

Werner, H.B.; Kuhlmann, K.; Shen, S.; Uecker, M.; Schardt, A.; Dimova, K.; Orfaniotou, F.; Dhaunchak, A.; Brinkmann, B.G.; Möbius, W.; Guarente, L.; Casaccia-Bonnefil, P.; Jahn, O.; Nave, K.-A. Proteolipid protein is required for transport of Sirtuin 2 into CNS myelin. J Neurosci 2007; 27:7717-7730.

Übersichtsartikel:

Jahn, O.; Tenzer, S.; Werner, H.B. Myelin proteomics: Molecular anatomy of an insulating sheath. Mol Neurobiol 2009; 40:55-72.

de Monasterio-Schrader, P.; Jahn, O.; Tenzer, S.; Wichert, S.P.; Patzig, J.; Werner, H.B. Systematic approaches to central nervous system myelin. Cell Mol Life Sci 2012; 69:2879-2894.


Proteomanalyse der Synapse

Ambrozkiewicz, M.C.; Borisova, E.; Schwark, M.; Ripamonti, S.; Schaub, T.; Smorodchenko, A.; Weber, A.I.; Rhee, H.J.; Altas, B.; Yilmaz, R.; Mueller, S.; Piepkorn, L.; Horan, S.T.; Straussberg, R.; Zaqout, S.; Jahn, O.; Dere, E.; Rosário, M.; Boehm-Sturm, P.; Borck, G.; Willig, K.I.; Rhee, JS.; Tarabykin, V.; Kawabe, H. The murine ortholog of Kaufman oculocerebrofacial syndrome protein Ube3b regulates synapse number by ubiquitinating Ppp3cc. Mol Psychiatry. 2020 Apr 6. doi: 10.1038/s41380-020-0714-8.

Ambrozkiewicz, M.C.; Schwark, M.; Kishimoto-Suga, M.; Borisova, E.; Hori, K.; Salazar-Lázaro, A.; Rusanova, A.; Altas, B.; Piepkorn, L.; Bessa, P.; Schaub, T.; Zhang, X.; Rabe, T.; Ripamonti, S.; Rosário, M.; Akiyama, H.; Jahn, O.; Kobayashi, T.; Hoshino, M.; Tarabykin, V.; Kawabe, H. Polarity acquisition in cortical neurons is driven by synergistic action of Sox9-regulated Wwp1 and Wwp2 E3 ubiquitin ligases and intronic miR-140. Neuron 2018; 100:1097-1115.

Altas, B.; Jahn, O.; Kawabe, H. Biochemical purification of binding partners of synaptic scaffold proteins. Methods Mol Biol 2017; 1538:69-82.

Biesemann, C.; Grønborg, M.; Luquet, E.; Wichert, S.P.; Bernard, V.; Bungers, S.R.; Cooper, B.; Varoqueaux, F.; Li, L.; Byrne, J.A.; Urlaub, H.; Jahn, O.; Brose, N.; Herzog, E. Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting. EMBO J 2014; 33:157-170.

Kawabe, H.; Neeb, A.; Dimova, K.; Young, S.M.; Takeda, M.; Katsurabayashi, S.; Mitkovski, M.; Malakhova, O.A.; Zhang, D.-E.; Umikawa, M.; Kariya, K.; Goebbels, S.; Nave, K.-A.; Rosenmund, C.; Jahn, O.; Rhee, J.-S.; Brose, N. Regulation of Rap2A by the ubiquitin ligase Nedd4-1 controls neurite development. Neuron 2010; 65:358-372.


Synaptische Calmodulin-Targets & Photoaffinitätsmarkierung

Piotrowski, C.; Moretti, R.; Ihling, C.H.; Haedicke, A.; Liepold, T.; Lipstein, N.; Meiler, J.; *Jahn, O.; Sinz, A. Delineating the molecular basis of the Calmodulin/bMunc13-2 interaction by cross-linking/mass spectrometry - Evidence for a novel CaM binding motif in bMunc13-2. Cells 2020; 9:136 (*co-corresponding author).

Akula, A.K.; Zhang, X.; Viotti, J.S.; Nestvogel, D.; Rhee, J.-S.; Ebrecht, R.; Reim, K.; Wouters, F.; Liepold, T.; Jahn, O.; Bogeski, I.; Dresbach, T. The Calmodulin binding region of the synaptic vesicle protein Mover is required for homomeric interaction and presynaptic targeting. Front Mol Neurosci 2019; 12:249.

Lipstein, N., Schaks, S., Dimova, K., Kalkhof, S., Ihling, C., Kölbel, K., Ashery, U., Rhee, J., Brose, N., Sinz, A., Jahn, O. Nonconserved Ca2+/calmodulin binding sites in Munc13s differentially control synaptic short-term plasticity. Mol Cell Biol 2012; 32:4628-4641.

Rodriguez-Castaneda, F.; Maestre-Martinez, M.; Coudevylle, N.; Dimova, K.; Junge, H.; Lipstein, N.; Lee, D.; Becker, S.; Brose, N.; Jahn, O.; Carlomagno, T.; Griesinger, C. Modular architecture of Munc13/calmodulin complexes: Dual regulation by Ca2+ and possible function in short-term synaptic plasticity. EMBO J 2010; 29:680-691.

Dimova, K.; Kalkhof, S.; Pottratz, I.; Ihling, C.; Rodriguez-Castaneda, F.; Liepold, T.; Griesinger, C.; Brose, N.; Sinz, A.; Jahn, O. Structural insights into the calmodulin - Munc13 interaction obtained by cross-linking and mass spectrometry. Biochemistry 2009; 48:5908-5921.

Dimova, K.; Kawabe, H.; Betz, A.; Brose, N.; Jahn, O. Characterization of the Munc13-calmodulin interaction by photoaffinity labeling. Biochim Biophys Acta 2006; 1763:1256-1265.

Übersichtsartikel:

Lipstein, N.; Göth, M.; Piotrowski, C.; Pagel, K.; Sinz, A.; Jahn, O. Presynaptic Calmodulin targets: lessons from structural proteomics. Expert Rev Proteomics 2017; 14:223-242.


Charakterisierung von Beta-Amyloid-Varianten

Klafki, H.W.; Rieper, P.; Matzen, A.; Zampar, S.; Wirths, O.; Vogelgsang, J.; Osterloh, D.; Rohdenburg, L.; Oberstein, T.J.; Jahn, O.; Beyer, I.; Lachmann, I.; Knölker, H.-J.; Wiltfang, J. Development and technical validation of an immunoassay for the detection of APP669-711 (Aβ-3-40) in biological samples. Int J Mol Sci 2020; 21:6564.

Hornung, K.; Zampar, S.; Engel, N.; Klafki, H.; Liepold, T.; Bayer, T.A.; Wiltfang, J.; Jahn, O.; Wirths, O. N-terminal truncated Aβ4-42 is a substrate for neprilysin degradation in vitro and in vivo. J Alzheimers Dis 2019; 67:849-858.

Beyer, I.; Rezaei-Ghaleh, N.; Klafki, H.-W.; Jahn, O.; Haußmann, U.; Wiltfang, J.; Zweckstetter, M.; Knölker, H.-J. Solid-phase synthesis and characterization of N-terminally elongated Aβ−3–x-peptides. Chemistry 2016; 22:8685-8693.

Haußmann, U.; Jahn, O.; Linning, P.; Janßen, C.; Liepold, T.; Portelius, E.; Zetterberg, H.; Bauer, C.; Schuchhardt, J.; Knölker, H.-J.; Klafki, H.; Wiltfang, J. Analysis of amino-terminal variants of amyloid-β peptides by capillary isoelectric focussing immunoassay. Anal Chem 2013; 85:8142-8149.

Schieb, H.; Kratzin, H.; Jahn, O.; Moebius, W.; Rabe, S.; Staufenbiel, M.; Wiltfang, J.; Klafki, H.W. β-Amyloid peptide variants in brains and cerebrospinal fluid from APP transgenic mice: Comparison with human Alzheimer's amyloid. J Biol Chem 2011; 286:33747-33758.


Methodenentwicklung

Kusch, K.; Uecker, M.; Liepold, T.; Möbius, W.; Hoffmann, C.; Neumann, H.; Werner, H.B.; Jahn, O. Partial immunoblotting of 2D-gels: A novel method to identify post-translationally modified proteins exemplified for the myelin acetylome. Proteomes 2017; 5:3.

Kuharev, J.; Navarro, P.; Distler, U.; Jahn, O.; Tenzer, S. In-depth evaluation of software tools for data-independent acquisition-based label-free quantification. Proteomics 2015; 15:3140-3151.

Pettelkau, J.; Ihling, C.H.; Frohberg, P.; van Werven, L.; *Jahn, O.; Sinz, A. Reliable identification of cross-linked products in protein interaction studies by 13C-labeled p-benzoylphenylalanine. J Am Soc Mass Spectrom 2014; 25:1628-1641 (*co-corresponding author).

Schmidt, C.; Hesse, D.; Raabe, M.; Urlaub, H.; Jahn, O. An automated in-gel digestion / iTRAQ-labeling workflow for robust quantification of gel-separated proteins. Proteomics 2013; 13:1417-1422.

Jahn, O.; Hesse, D.; Reinelt, M.; Kratzin, H.D. Technical innovations for the automated identification of gel-separated proteins by MALDI-TOF mass spectrometry. Anal Bioanal Chem 2006; 386:92-103.

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