Publications

  1. Willig, K.I. §, Wegner, W., Müller, A., Clavet-Fournier, V., and Steffens, H. (2021). Multi-label in vivo STED microscopy by parallelized switching of reversibly switchable fluorescent proteins. Cell Reports 35, 109192.
  2. Steffens, H., Mott, A.C., Li, S., Wegner, W., Švehla, P., Kan, V.W.Y., Wolf, F., Liebscher, S., and Willig, K.I. (2021). Stable but not rigid: Chronic in vivo STED nanoscopy reveals extensive remodeling of spines, indicating multiple drivers of plasticity. Science Advances 7, eabf2806.
  3. Spieth L., Berghoff S.A., Stumpf S.K., Winchenbach J., Michaelis T., Watanabe T., Gerndt N., Düking T., Hofer S., Ruhwedel T., Shaib A.H., Willig K., Kronenberg K., Karst U., Frahm J., Rhee J.S., Minguet S., Möbius W., Kruse N., von der Brelie C., Michels P., Stadelmann C., Hülper P., Saher G. (2021). “Anesthesia triggers drug delivery to experimental glioma in mice by hijacking caveolar transport” Neuro-Oncology Adv 1–35. doi:10.1093/noajnl/vdab140
  4. Kuljis D.A., Micheva K.D., Ray A., Wegner W., Bowman R., Madison D. V., Willig K.I., Barth A.L.. (2021). Gephyrin-Lacking PV Synapses on Neocortical Pyramidal Neurons. Int J Mol Sci 22, 10032. doi:10.3390/ijms221810032
  5. Götze, T., Soto-Bernardini, M.C., Zhang, M., Mießner, H., Linhoff, L., Brzózka, M.M., Velanac, V., Dullin, C., Ramos-Gomes, F., Peng, M.,…Willig, K.,…Schwab, M.H. (2021). “Hyperactivity is a Core Endophenotype of Elevated Neuregulin-1 Signaling in Embryonic Glutamatergic Networks.” Schizophr. Bull. 1–12.
  6. Badowska, D.M., M.M. Brzózka, N. Kannaiyan, C. Thomas, P. Dibaj, A. Chowdhury, H. Steffens, C.W. Turck, P. Falkai, A. Schmitt, S. Papiol, V. Scheuss, K.I. Willig, D. Martins-de-Souza, J.S. Rhee, D. Malzahn, M.J. Rossner (2020): “Modulation of cognition and neuronal plasticity in gain- and loss-of-function mouse models of the schizophrenia risk gene Tcf4” Transl. Psychiatry 10, 343.
  7. Ambrozkiewicz, M.C., E. Borisova, M. Schwark, S. Ripamonti, T. Schaub, A. Smorodchenko, A.I. Weber, H. J. Rhee, B. Altas, R. Yilmaz, …, K.I. Willig, JS Rhee, V. Tarabykin, H. Kawabe (2020): “The murine ortholog of Kaufman oculocerebrofacial syndrome protein Ube3b regulates synapse number by ubiquitinating Ppp3cc.” Mol. Psychiatry https://doi.org/10.1038/s41380-020-0714-8
  8. Steffens, H., W. Wegner, K.I. Willig (2020): “In vivo STED microscopy: a roadmap to nanoscale imaging in the living mouse.“ Methods 174, 42-48
  9. Stahlberg M. A., C. Ramakrishnan, K.I. Willig, E.S. Boyden, K. Deisseroth, C. Dean (2019): “Investigating the feasibility of channelrhodopsin variants for nanoscale optogenetics” Neurophotonics 6 (2019) 1.
  10. Wegner W., A.C. Mott, S.G.N. Grant, H. Steffens, K.I. Willig (2018): “In vivo STED microscopy visualizes PSD95 sub-structures and morphological changes over several hours in the mouse visual cortex.” Scientific Reports 8, 219-219
  11. Neef, J., N. T. Urban§, T.-L. Ohn, T. Frank, P. Jean, S. W. Hell, K. I. Willig§, T. Moser§ (2018) “Quantitative optical nanophysiology of Ca2+ signaling at inner hair cell active zones.” Nature Commun. 9, 290
  12. Wegner W., P. Ilgen, C. Gregor, J. van Dort, A.C. Mott, H. Steffens, K.I. Willig (2017): “In vivo mouse and live cell STED microscopy of neuronal actin plasticity using far-red emitting fluorescent proteins.” Scientific Reports 7, 11781-11781
  13. Richardson D.S., C. Gregor, F.R. Winter, N.T. Urban, S.J. Sahl, K.I. Willig, S.W. Hell (2017): “SRpHi ratiometric pH biosensors for super-resolution microscopy”. Nat. Commun. 8, 577-577
  14. Hell, S. W., S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, T. Cordes (2015): "The 2015 super-resolution microscopy roadmap" J. Phys. D: Appl. Phys. 48, 443001
  15. Eggeling, C., K. I. Willig, S. J. Sahl, S. W. Hell (2015): "Lens-based fluorescence nanoscopy" Q. Rev. Biophys. 48, 178-243
  16. Willig, K. I., F. J. Barrantes (2014): "Recent applications of superresolution microscopy in neurobiology" Curr. Opin. Chem. Biol. 20, 16 – 21
  17. Willig, K. I.§, H. Steffens, C. Gregor, A. Herholt, M. J. Rossner, S. W. Hell§ (2014): "Nanoscopy of filamentous actin in cortical dendrites of a living mouse" Biophysical Journal 106, L01 - L03
  18. Agarwal, A., Zhang, M., Trembak-Duff, I., Unterbarnscheidt, T., Radyushkin, K., Dibaj, P., … Willig, K. I.,… Schwab, M. H. (2014). Dysregulated Expression of Neuregulin-1 by Cortical Pyramidal Neurons Disrupts Synaptic Plasticity. Cell Reports.
  19. Jensen, N. A., J. G. Danzl, K. I. Willig, F. Lavoie-Cardinal, T. Brakemann, S. W. Hell, S. Jakobs (2014): "Coordinate-Targeted and Coordinate-Stochastic Super- Resolution Microscopy with the Reversibly Switchable Fluorescent Protein Dreiklang" ChemPhysChem 15, 756 – 762
  20. Belov, V. N., G. Y. Mitronova, M. L. Bossi, V. P. Boyarskiy, E. Hebisch, C. Geisler, K. Kolmakov, C. A. Wurm, K. I. Willig, S. W. Hell (2014): "Masked Rhodamine Dyes of Five Principal Colors Revealed by Photolysis of a 2-Diazo-1-Indanone Caging Group: Synthesis, Photophysics, and Light Microscopy Applications" Chem. Eur. J. 20, 13162-13173
  21. Eggeling, C., K. I. Willig, F. J. Barrantes (2013): "STED microscopy of living cells - new frontiers in membrane and neurobiology", J. Neurochem. 126, 203 – 212
  22. Berning, S., K. I. Willig§, H. Steffens, P. Dibaj, S. W. Hell§ (2012): "Nanoscopy in a Living Mouse Brain". Science 335, 551 
  23. Testa, I., N. T. Urban, S. Jakobs, C. Eggeling, K. I. Willig, S. W. Hell (2012): "Nanoscopy of living brain slices with low light levels" Neuron 75, 992 – 1000
  24. Nizamov, S., K. I. Willig, M. V. Sednev, V. N. Belov, S. W. Hell (2012): "Phosphorylated 3-Heteroarylcoumarins and their use in fluorescence microscopy and nanoscopy" Chemistry - A European Journal 18, 16339 - 16348
  25. Willig, K. I. § , A. C. Stiel, T. Brakemann, S. Jakobs, S. W. Hell§ (2011): "Dual-label STED nanoscopy of living cells using photochromism" Nano Lett. 11, 3970 – 3973
  26. Urban, N., K. I. Willig, S. W. Hell, U. V. Nägerl (2011): "STED Nanoscopy of Actin Dynamics in Synapses Deep Inside Living Brain Slices" Biophys. J. 101, 1277-1284
  27. Lalkens, B., I. Testa, K. I. Willig, S. W. Hell: "MRT Letter (2011): Nanoscopy of Protein Colocalization in Living Cells by STED and GSDIM" Micr. Res. Techn. 75, 1 – 6
  28. Grotjohann, T., I. Testa, M. Leutenegger, H. Bock, N. T. Urban, F. Lavoie-Cardinal, K. I. Willig, C. Eggeling, S. Jakobs, S. W. Hell (2011): "Diffraction-unlimited all-optical imaging and writing with a photochromic GFP" Nature 478, 204 – 208
  29. van den Bogaart, G., K. Meyenberg, H. Jelger Risselada, H. Amin, K. I. Willig, B. E. Hubrich, M. Dier, S. W. Hell, H. Grubmüller, U. Diederichsen, R. Jahn (2011): "Membrane protein sequestering by ionic protein-lipid interactions" Nature 479, 552-555
  30. Watanabe, S., A. Punge, G. Hollopeter, K. I. Willig, R. J. Hobson, M. W. Davis, S. W. Hell, E. M. Jorgensen (2011): "Protein localization in electron micrographs using fluorescence nanoscopy" Nature Meth. 8, 80-84
  31. Kleine-Vehn, J., K. Wabnik, A. Martinière, L. Langowski, K. Willig, S. Naramoto, J. Leitner, H. Tanaka, S. Jakobs, S. Robert, C. Luschnig, W. Govaerts, S. W. Hell, J. Runions, J. Friml (2011): "Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at the plasma membrane" Molecular Systems Biology 7, 1 – 13
  32. Tønnesen, J., F. Nadrigny, K.I. Willig, R. Wedlich-Söldner, U.V. Nägerl (2011): “Two-Color STED Microscopy of Living Synapses Using A Single Laser-Beam Pair”. Biophys. J. 101 (2011) 2545–2552.
  33. Hein B., K. I. Willig, C. A. Wurm, V. Westphal, S. Jakobs, S. W. Hell (2010): "Stimulated emission depletion nanoscopy of living cells using SNAP-Tag fusion proteins". Biophys. J. 98, 158 - 163.
  34. Han, K. Y., K. I. Willig, E. Rittweger, F. Jelezko, C. Eggeling, S. W. Hell (2009): "Three-dimensional stimulated emission depletion microscopy of nitrogen-vacancy centers in diamond using continuous-wave light". Nano Lett. 9 (9), 3323 - 3329.
  35. Hein, B., K. I. Willig, S. W. Hell (2008): "Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell". PNAS 105 (38), 14271-14276.
  36. Nägerl, U. V.*, K. I. Willig*, B. Hein, S. W. Hell, T. Bonhoeffer (2008): "Live-cell imaging of dendritic spines by STED microscopy". PNAS 105 (48), 18982-18987
  37. Willig, K. I.*, B. Harke*, R. Medda, S. W. Hell (2007): "STED microscopy with continuous wave beams". Nature Methods 4 (11), 915 - 918. 
  38. Sieber, J. J., K. I. Willig, C. Kutzner, C. Gerding-Reimers, B. Harke, G. Donnert, B. Rammner, C. Eggeling, S. W. Hell, H. Grubmüller, T. Lang (2007): "Anatomy and dynamics of a supramolecular membrane protein cluster". Science 317: 1072 – 1076.
  39. Kellner, R, J. Baier, K. I. Willig, S. W. Hell, F. J. Barrantes (2007): "Nanoscale organization of nicotinic acetylcholine receptors revealed by STED microscopy". Neuroscience 144 (1), 135 – 143.
  40. Willig, K. I.*, S. O. Rizzoli*, V. Westphal, R. Jahn, S. W. Hell (2006): "STED-microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis". Nature 440 (7086), 935-939.
  41. Willig, K. I., R. R. Kellner, R. Medda, B. Hein, S. Jakobs, S. W Hell (2006): "Nanoscale resolution in GFP-based microscopy". Nature Methods (9), 721-723.
  42. Willig, K. I., J. Keller, M. Bossi, S. W. Hell (2006): "STED microscopy resolves nanoparticle assemblies". New J. Phys. 8, 106.
  43. Sieber, J. J., K. I. Willig, R. Heintzmann, S. W. Hell, T. Lang (2006): “The SNARE-motif is essential for the formation of syntaxin clusters in the plasma membrane”. Biophys. J. 90, 2843-2851.
  44. Kittel, R. J., C. Wichmann, T. M. Rasse, W. Fouquet, M. Schmidt, A. Schmid, D. A. Wagh, C. Pawlu, R. R. Kellner, K. I. Willig, S. W. Hell, E. Buchner, M. Heckmann, S. J. Sigrist (2006): "Bruchpilot promotes active zone assembly, Ca2+ -channel clustering, and vesicle release". Science 312, 1051-1054.
  45. Fitzner, D., A. Schneider, A. Kippert, W. Möbius, K. I. Willig, S. W. Hell, G. Bunt, K. Gaus, M. Simons (2006): "Myelin basic protein-dependent plasma membrane reorganization in the formation of myelin". EMBO J. 25, 5037 – 5048.
  46. Hell S.W., Willig K.I., Dyba M., Jakobs S., Kastrup L., Westphal V. (2006) Nanoscale Resolution with Focused Light: Stimulated Emission Depletion and Other Reversible Saturable Optical Fluorescence Transitions Microscopy Concepts. In: Pawley J. (eds). Handbook Of Biological Confocal Microscopy. Springer, Boston, MA  Online ISBN 978-0-387-45524-2
  47. Merschdorf M., C. Kennerknecht, K. Willig, W. Pfeiffer (2002): „Transient electron energy distribution in supported Ag nanoparticles“. New J. Phys. 4, 95
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